Bradford Assay for Determining Protein Concentration
Abstract
The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.
Footnotes
-
From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
