Detecting β-Galactosidase-Labeled Cells
Abstract
β-Galactosidase has been used extensively both as a label in enzyme immunoassays and for immunocytochemistry. One good substrate is 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal), which gives an intense blue product. The product is stable and insoluble in alcohol as well as H2O.
Footnotes
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From the Antibodies collection, edited by Edward A. Greenfield.
