GYT Medium
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Glycerol | 10 mL | 10% (v/v) |
| Yeast extract | 0.125 g | 0.125% (w/v) |
| Tryptone | 0.25 g | 0.25% (w/v) |
| Sterilize the medium by passing it through a prerinsed 0.22-µm filter. Store in 2.5-mL aliquots at 4°C. | ||
