Recipe

IMAC Regeneration Buffer

Reagent Quantity (for 100 mL) Final concentration
Guanidinium chloride   57.3 g   6 m
Acetic acid, glacial (17.5 m) 1.14 mL 0.2 m
Store at room temperature.
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  1. doi:10.1101/pdb.rec105437 Cold Spring Harb Protoc 2020: pdb.rec105437- © 2020 Cold Spring Harbor Laboratory Press

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