IMAC Imidazole Elution Buffer
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Sodium phosphate (pH 7.4) | 1.13 g of sodium phosphate monobasic, anhydrous and 5.75 g of sodium phosphate dibasic, anhydrous | 50 mm |
| NaCl | 29.22 g | 500 mm |
| Imidazole (250 mm) | 17.0 g | 250 mm |
| Store at 4°C. | ||
