Protocol

Purification of Labeled Antibodies Using Size-Exclusion Chromatography

  1. Jordan B. Fishman

Abstract

Many antibody labeling procedures call for a desalting or purification step requiring size-exclusion chromatography (SEC). The method outlined here contains information needed to desalt an antibody conjugate. Similar procedures would be used for ion-exchange chromatography using a gradient of increasing ionic strength. Resins can be purchased in bulk (as in this protocol), or commercially available columns are available.

Footnotes

  • From the Antibodies collection, edited by Edward A. Greenfield.

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  1. doi:10.1101/pdb.prot099358 Cold Spring Harb Protoc 2020: pdb.prot099358- © 2020 Cold Spring Harbor Laboratory Press
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