Table 1.
SEC Sephadex resins and the effective separation range of each
| Sephadex | MW range | Application |
|---|---|---|
| G-10 | ≤700 | Very-low MW substances; desalting; buffer exchange: peptides |
| G-15 | ≤1500 | Very-low MW substances; desalting; buffer exchange: peptides |
| G-25 | 1000–5000 | Most common for desalting; buffer exchange: small peptides and proteins |
| G-50 | 1500–30,000 | Desalting; buffer exchange: small peptides and proteins |
| G-75 | 30,000–80,000 | Protein separation |
| G-100 | 4000–150,000 | Protein conjugate separation |
| G-150 | 5000–300,000 | Protein conjugate separation; protein complexes—low noncovalent interactions |
| G-200 | 5000–600,000 | Protein conjugate separation; protein complexes—low noncovalent interactions |
