Binding Antibodies to Attached Cells or Tissues in Preparation for Staining

Abstract

Cells for staining are usually prepared from one of three sources: adherent cells, suspension cells, or whole tissues. Antibodies generally are applied directly to the area of the cells or tissues that is being studied. The antibodies can be labeled directly or they can be detected by using a labeled secondary reagent that will bind specifically to the primary antibody. Detection reagents for cell staining can be labeled with fluorochromes, enzymes, gold, or iodine.