Paraformaldehyde Stock Solution (4% in PBS)
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1. Add 8 g of paraformaldehyde to 100 mL of H2O.
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2. Heat to 60°C in a fume hood. Add a few drops of 1 n NaOH to help it dissolve.
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Paraformaldehyde dissolves in water, releasing formaldehyde in the process; thus preparation in a fume hood is essential.
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3. When the solid has completely dissolved, let the solution cool to room temperature, and add 100 mL of 2× PBS.
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This stock solution should be prepared fresh daily.
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