Formaldehyde Solution (11%)
| Reagent | Volume per 50 mL of solution (v/v) | Final concentration |
|---|---|---|
| HEPES-KOH (1 m, pH 7.5) | 2.5 mL | 50 mm |
| NaCl (5 m) | 1.0 mL | 100 mm |
| EDTA (0.5 m) | 100 µL | 1 mm |
| EGTA (0.5 m) | 50 µL | 0.5 mm |
| Formaldehyde (37%) | 14.9 mL | 11% |
| ddH2O | Add to 100 mL | |
| Store at room temperature. | ||
