Lysis Buffer 3 for ChIP
| Reagent | Volume per 100 mL of solution (v/v) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 8.0) | 1.0 mL | 10 mm |
| NaCl (5 m) | 2.0 mL | 100 mm |
| EDTA (0.5 m) | 0.2 mL | 1 mm |
| EGTA (0.5 m) | 0.1 mL | 0.5 mm |
| Na-deoxycholate (10%) | 1.0 mL | 0.1% |
| N-Lauroyl-sarcosine (20%) | 2.5 mL | 0.5% |
| ddH2O | Add to 100 mL | |
| Store at 4°C. | ||
