Recipe

His-Fusion Lysis Buffer (pH 7.4)

Reagent Final concentration
Imidazole  20 mm
Sodium chloride 300 mm
Sodium phosphate  50 mm
Protease inhibitor tablets, EDTA-free (Roche)
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  1. doi:10.1101/pdb.rec101931 Cold Spring Harb Protoc 2020: pdb.rec101931- © 2020 Cold Spring Harbor Laboratory Press
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