Recipe

Inclusion Body Lysis Buffer

Reagent Final concentration
EDTA 1 mm
Dithiothreitol 5 mm
Phosphate-buffered saline (pH 7.4)
Protease inhibitor tablets, EDTA-free (Roche)
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  1. doi:10.1101/pdb.rec101972 Cold Spring Harb Protoc 2020: pdb.rec101972- © 2020 Cold Spring Harbor Laboratory Press
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