Prehybridization Solution for Northerns
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Na2HPO4·7H2O | 134 g | 0.5 m |
| H3PO4 (concentrated phosphoric acid, 85%) | 4 mL | |
| SDS | 70 g | 7% (w/v) |
| EDTA (0.5 m, pH 7.0) | 2 mL | 1 mm |
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Na2HPO4·7H2O | 134 g | 0.5 m |
| H3PO4 (concentrated phosphoric acid, 85%) | 4 mL | |
| SDS | 70 g | 7% (w/v) |
| EDTA (0.5 m, pH 7.0) | 2 mL | 1 mm |