Recipe

Prehybridization Solution for Northerns

Reagent Quantity (for 1 L) Final concentration
Na2HPO4·7H2O 134 g   0.5 m
H3PO4 (concentrated phosphoric acid, 85%) 4 mL
SDS  70 g 7% (w/v)
EDTA (0.5 m, pH 7.0) 2 mL   1 mm
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  1. doi:10.1101/pdb.rec104372 Cold Spring Harb Protoc 2021: pdb.rec104372- © 2021 Cold Spring Harbor Laboratory Press
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