Bleaching Solution for In Situ Hybridization
| Reagent | Volume (for 100 mL) | Final concentration |
|---|---|---|
| Sodium chloride-sodium citrate buffer (SSC; 20×) | 2.5 mL | 0.5× |
| Formamide | 5 mL | 5% |
| Peroxide (30%) | 4 mL | 1.2% |
| H2O | 88.5 mL |
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Add the formamide and peroxide to the larger volume of SSC, in that order only; formamide and peroxide can become explosive if mixed directly together. This solution must be made fresh. Do not store.
