Protocol

One-Step Preparation of Competent E. coli: Transformation and Storage of Bacterial Cells in the Same Solution

  1. Joseph Sambrook

Abstract

This protocol describes a convenient method for the preparation, use, and storage of competent Escherichia coli. The reported transformation efficiency of this method is ∼5 × 107 transformants/µg of plasmid DNA.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. doi:10.1101/pdb.prot101212 Cold Spring Harb Protoc 2021: pdb.prot101212- © 2021 Cold Spring Harbor Laboratory Press
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