Recipe

TSS Solution

Prepare LB medium (pH 6.1) containing 10% PEG (MW ∼ 3350), 10 mm MgCl2, and 10 mm MgSO4. Adjust the pH of the final solution to pH 6.5 and store at 4°C.

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  1. doi:10.1101/pdb.rec103440 Cold Spring Harb Protoc 2021: pdb.rec103440- © 2021 Cold Spring Harbor Laboratory Press
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