Protocol

Variations of Staining Sodium Dodecyl Sulfate–Polyacrylamide Gels with Coomassie Brilliant Blue

Abstract

Many variations of the original Coomassie Brilliant Blue staining procedure are in use. This protocol describes some selected variations on the standard procedure that give comparable and consistent staining results for proteins in the 20- to 200-kDa range.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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