AE Buffer for RNA Isolation
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| Sodium acetate (3 m, pH 5.2) | 1.67 mL | 50 mm |
| EDTA (0.5 m) | 2 mL | 10 mm |
| Bring the final volume to 100 mL with DEPC-treated H2O. This buffer should be sterilized before use by autoclaving for 15 min at 15 lb/in2 for 20 min, and stored at room temperature. | ||
