| Tris base |
30.3 g |
250 mm |
25 mm |
| Glycine, electrophoresis grade |
144 g |
1.9 m |
192 mm |
| SDS, electrophoresis grade |
10 g |
1% |
0.1% (w/v) |
| H2O
|
to 1 L |
|
|
| The pH should be ∼8.3. |
| Several manufacturers sell electrophoresis-grade SDS. Although any one of these will give reproducible results, they are not
interchangeable, and we recommend exclusive use of a single brand of SDS because migration of polypeptides may change noticeably
when one brand of SDS is substituted for another. If proteins are to be eluted from the gel for sequencing, electrophoresis-grade
SDS should be further purified as described by Hunkapiller et al. (1983). A 20% (w/v) stock solution of SDS should be prepared in deionized H2O and stored at room temperature.
|
