How to Grow Xenopus laevis Tadpole Stages to Adult

Abstract

In Xenopus laevis, the tadpole stage is characterized by three forms—those occurring before the initiation of limb development, those covering limb development, and those encompassing metamorphosis. Maximal tadpole growth, especially during the second form, is critically dependent on good husbandry practices. Here we describe a protocol for raising Xenopus laevis tadpoles through to adulthood. Each step may need to be modified depending on the aquaria used and local conditions.