| Human chorionic gonadotropin (hCG; from National Hormone and Peptide Program, Sigma-Aldrich, Merck Animal Health [Chorulon
brand], or BioVendor)
|
1000 U/mL |
| Ovine luteinizing hormone (oLH; from National Hormone and Peptide Program) |
0.4 mg/mL |
| Pregnant mare serum gonadotropin (PMSG; from BioVendor) |
100 U/mL |
| Dissolve phosphate-buffered saline (PBS) tablets (Sigma-Aldrich P4417) in type 1 ultrapure water, at a concentration of 1
tablet per 200 mL. Sterilize by autoclaving. Prepare individual hormone solutions in 1× PBS at the concentrations listed above.
Store all hormone solutions at 20°C until needed for use.
|
