Recipe

Culture Media Base

Reagent Amount Final concentration
L-15 medium (Sigma-Aldrich L1518)  98 mL 49%
Ringer's solution (1×) 100 mL 50%
Sterilize by vacuum filtration. Add 2 mL of Antibiotic-Antimycotic (100×; Gibco 15240062). Store at 4°C.
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  1. doi:10.1101/pdb.rec107136 Cold Spring Harb Protoc 2021: pdb.rec107136- © 2021 Cold Spring Harbor Laboratory Press
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