Plating Culture Medium for Neural Crest Cells
| Reagent | Amount | Final concentration |
|---|---|---|
| NaCl | 1.55 g | 53 mm |
| Na2CO3 | 0.265 g | 5 mm |
| Potassium gluconate | 0.527 g | 4.5 mm |
| Sodium gluconate | 3.82 g | 35 mm |
| MgSO4 | 0.060 g | 1 mm |
| CaCl2 | 0.055 g | 1 mm |
| Add reagents to 400 mL of deionized (DI) H2O. Adjust the pH to 8.0 with bicine, and bring to final volume (500 mL) with DI H2O. Sterilize by filtration. Store frozen at −20°C in small aliquots. Add 50 µg/mL gentamicin sulfate and 1 µg/mL bovine serum albumin to the culture medium prior to use. | ||
