Steinberg's Medium
| Reagent | Amount | Final concentration |
|---|---|---|
| NaCl | 3.39 g | 58 mm |
| KCl | 0.05 g | 0.67 mm |
| Ca(NO3)2 | 0.07 g | 0.44 mm |
| MgSO4 | 0.16 g | 1.3 mm |
| Tris (1 m, pH 7.8) | 2.3 mL | 4.6 mm |
| Add reagents to 250 mL of deionized (DI) H2O and adjust the pH to 7.8. Fill to final volume (500 mL) with DI H2O. Sterilize by autoclaving. Store indefinitely at room temperature. | ||
