Plasticine Clay-Coated Petri Dishes
To prepare dissection dishes, gather several 35-mm or 60-mm tissue-culture dishes (e.g., Falcon 353001). Push a small amount of plasticine clay (e.g., Plastilina Modeling Clay) into the middle of each dish, and then spread it evenly to fill the bottom 3–5 mm. Rinse the surface of the clay with ample water and ethanol to remove excess oily residue. UV-sterilize prior to use.
After the dissections, sterilize the dishes for reuse. Rinse them thoroughly with deionized H2O to remove any embryo particulate, use a gloved hand to level out the depressions made for embryos, rinse with ethanol, and sterilize under UV light. Cap and store with dissection kit. Discard and make fresh dissection dishes if clay appears dirty or has trapped any debris.
