Recipe

Tadpole-Rearing Medium

15 mm NaCl

0.5 mm KCl

1.0 mm MgSO4

150 µm KH2PO4

50 µm NaHPO4

1.0 mm CaCl2

0.7 mm NaHCO3

0.5 mg/L methylene blue

Prepare as needed in deionized H2O, and check that the pH is 7.2–7.6 before use.

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  1. doi:10.1101/pdb.rec107300 Cold Spring Harb Protoc 2021: pdb.rec107300- © 2021 Cold Spring Harbor Laboratory Press
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