Protocol

Mapping RNA with Nuclease S1

  1. Joseph Sambrook

Abstract

This protocol provides details for nuclease S1 mapping of mRNA using a uniformly labeled, single-stranded DNA probe. DNA–RNA hybrids are generated, which are subsequently digested with nuclease S1. The digestion products are separated using gel electrophoresis and analyzed by radiography.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. doi:10.1101/pdb.prot101824 Cold Spring Harb Protoc 2021: pdb.prot101824- © 2021 Cold Spring Harbor Laboratory Press
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