Primer Extension Mix for RNA Analysis
| Reagent | Quantity (for 10 mL) | Final concentration |
|---|---|---|
| Tris-Cl (1 m, pH 8.4, at room temperature) | 200 µL | 20 mm |
| MgCl2 (1 m) | 100 µL | 10 mm |
| dNTP solution containing all four dNTPs (100 mm) | 160 µL | 1.6 mm |
| Actinomycin D (5 mg/mL) | 100 µL | 50 µg/mL |
| Dissolve actinomycin D in methanol at a concentration of 5 mg/mL before supplementing the primer extension mix. Store the actinomycin D stock solution at −20°C in the dark. | ||
