Topic Introduction

Mapping RNA

  1. Joseph Sambrook

Abstract

Details of the fine structure of a particular transcript may be assessed by S1 mapping or ribonuclease protection. The use of either of these techniques allows the detection of the 5′ and 3′ ends of a particular mRNA, as well as the splice junctions, precursors, and processing intermediates of mRNA. Primer extension provides a measure of the amount of a particular mRNA species and allows an exact determination of the 5′ end of the mRNA. These three methods are introduced here.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. doi:10.1101/pdb.top101816 Cold Spring Harb Protoc 2021: pdb.top101816- © 2021 Cold Spring Harbor Laboratory Press
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