Extracellular Bath Saline
| Reagent | Amount (for 1 L) | Final concentration |
|---|---|---|
| NaCl | 6.72 g | 115 mm |
| KCl | 0.30 g | 4 mm |
| CaCl2•2H2O | 0.44 g | 3 mm |
| MgCl2•6H2O | 0.61 g | 3 mm |
| HEPES | 1.19 g | 5 mm |
| Glycine | 0.00075 g | 0.01 mm |
| Glucose | 1.80 g | 10 mm |
| Tubocurarine | 0.068 g | 0.1 mm |
| To prepare 1 L of extracellular saline, dissolve the reagents listed above in 800 mL of ddH2O. Adjust the pH to 7.2 with NaOH, and then add ddH2O to 1 L. The final osmolarity is 255 mOsm. Store at 4°C. | ||
