Internal Recording Saline
| Reagent | Amount (for 20 mL) | Final concentration |
|---|---|---|
| Potassium gluconate | 515.4 mg | 110 mm |
| KCl | 11.9 mg | 8 mm |
| NaCl | 5.8 mg | 5 mm |
| MgCl2•6H2O | 6.1 mg | 1.5 mm |
| HEPES | 95.3 mg | 20 mm |
| EGTA | 3.8 mg | 0.5 mm |
| ATP | 44.1 mg | 4 mm |
| GTP | 3.1 mg | 0.3 mm |
| To prepare 20 mL of internal saline, dissolve the reagents listed above in 15 mL of ddH2O. Adjust the pH to 7.2 with KOH, and then add ddH2O to 20 mL. The final osmolarity is 255 mOsm. Store at −20°C, and thaw on ice just before the experiment. | ||
