Steinberg's Solution
| Reagent | Amount (for 100× stock) | Final concentration (for the 100× solution) |
|---|---|---|
| NaCl | 34 g | 582 mm |
| KCl | 0.5 g | 6.7 mm |
| Ca(NO3)2 • 4H2O | 0.8 g | 3.4 mm |
| MgSO4 • 7H2O | 2.06 g | 8.3 mm |
| HEPES | 119 g | 500 mm |
| To prepare 1 L of 100× Steinberg's solution, dissolve the reagents listed above in 800 mL of ddH2O. Adjust the pH to 7.5 with NaOH, and then add ddH2O to 1 L. Store the solution at 4°C. | ||
| To prepare 10 L of 1× Steinberg's solution, pour 100 mL of stock and add ddH2O to 10 L. Store at room temperature. | ||
