Purification of Labeled Oligonucleotides by Chromatography on a Sep-Pak C18 Column
Abstract
This protocol describes a method for the separation of radiolabeled oligonucleotides from unincorporated radiolabel that takes advantage of the reversible affinity of oligonucleotides for silica gel. This protocol can be used only to purify oligonucleotides carrying a 5′-phosphate group, radiolabeled or unlabeled.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
