Protocol

Cloning Polymerase Chain Reaction (PCR) Products: TOPO TA Cloning

  1. Joseph Sambrook

Abstract

This protocol describes the use of TOPO-activated TA vectors for cloning. Manufacturers of cloning kits provide excellent manuals that explain in detail what to do and why to do it. This makes TOPO cloning easy, but not foolproof. When setting up TOPO cloning for the first time, set up a trial experiment as described here.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. doi:10.1101/pdb.prot101311 Cold Spring Harb Protoc 2021: pdb.prot101311- © 2021 Cold Spring Harbor Laboratory Press
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