Azaserine/Hypoxanthine (AH) Selection Medium
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1. To prepare 100 mL of 100× AH, add 0.136 g of hypoxanthine in H2O suitable for tissue culture. Heat to 70°C to dissolve, and then add 10 mg of azaserine.
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The 100× stock is 0.58 mm azaserine and 10 mm hypoxanthine.
100× H will be needed for growing hybridoma cells while removing the azaserine selection. 100× H is prepared as above, but without the addition of azaserine.
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2. Filter-sterilize.
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3. Dispense into sterile tube in 2.0-mL aliquots. Store at −20°C.
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100× AH is stable when stored for 1 yr at −20°C.
Data from Foung et al. (1982).
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