Corrigendum: Combining the Dihydrofolate Reductase Protein-Fragment Complementation Assay with Gene Deletions to Establish Genotype-to-Phenotype Maps of Protein Complexes and Interaction Networks
Cold Spring Harb Protoc 2016; doi: 10.1101/pdb.prot090035
When this protocol was first published, the concentrations for the reagents in the recipe entitled “Amino Acid Mix (10×)” were expressed as g/mL, but they should have been expressed as g/L. The authors apologize for this error. The HTML version of the recipe (doi: 10.1101/pdb.rec090308) has been corrected, as has the current PDF version of the protocol. A corrected version of the recipe is also included below.
| Amino acid or nucleobase | Concentration (10×) |
|---|---|
| Adenine sulfate | 0.4 g/L |
| Uracil | 0.2 g/L |
| l-Tryptophan | 0.4 g/L |
| l-Histidine hydrochloride | 0.2 g/L |
| l-Arginine hydrochloride | 0.2 g/L |
| l-Tyrosine | 0.3 g/L |
| l-Leucine | 0.6 g/L |
| l-Lysine hydrochloride | 0.3 g/L |
| l-Phenylalanine | 0.5 g/L |
| l-Glutamic acid | 1.0 g/L |
| l-Asparagine | 1.0 g/L |
| l-Valine | 1.5 g/L |
| l-Threonine | 2.0 g/L |
| l-Serine | 3.75 g/L |
| l-Methionine | 0.2 g/L |
| Dissolve the amino acids from the list above (except those to be excluded from any drop-out media) in distilled H2O. Filter-sterilize, store at 4°C, and protect from light. | |
Amino Acid Mix (10×)
