Hybridoma Screening by Antigen Capture: Immunoprecipitation
Abstract
Immunoprecipitation is rarely used for screening hybridoma fusions because the assays are tedious and time-consuming. However, it can be useful when working with complex antigens because the precipitated antigen is normally detected after sodium dodecyl sulfate (SDS)–polyacrylamide electrophoresis and thus it is simple to discriminate between true and false positives. Furthermore, the assay provides information regarding the molecular weight of the antigen.
Footnotes
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From the Antibodies collection, edited by Edward A. Greenfield.
