Quick Transformation with Plasmid DNA
- 1Université Paris-Saclay, CEA, CNRS, Institut de Biologie Intégrative de la Cellule (I2BC), 91190 Gif-sur-Yvette, France
- 2Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41080 Sevilla, Spain
- ↵3Correspondence: lionello.bossi{at}i2bc.paris-saclay.fr
Abstract
Genomic engineering of Escherichia coli and Salmonella often requires introducing plasmids into strains obtained during the intermediate stages of the process. Such strains are typically transformed only once, making the preparation of large batches of competent cells for storage purposes unnecessary. Here, we describe a simple scaled-down procedure for transforming E. coli or Salmonella with plasmid DNA that uses as little as 2 mL of culture.
Footnotes
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From the Experiments in Bacterial Genetics collection, by Lionello Bossi, Andrew Camilli, and Angelika Gründling.
