Protocol

A Simple Method for Immunohistochemistry and Imaging of Mosquito Salivary Glands

  1. Eric Calvo1,3
  1. 1Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
  2. 2Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
  1. ↵3Correspondence: ecalvo{at}niaid.nih.gov

Abstract

Immunohistochemistry is a valuable technique that provides information on protein localization and interactions in tissues. Mosquito salivary gland immunohistochemistry requires the meticulous dissection of a delicate tissue. The integrity of the salivary glands must be closely monitored throughout the entire process to prevent structural damage and loss of saliva. This protocol describes a series of simple steps to perform salivary gland immunohistochemistry including tissue dissection, permeabilization, immunostaining, mounting, and imaging by confocal microscopy.

Footnotes

  • From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.

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This Article

  1. Published in Advance August 5, 2022, doi: 10.1101/pdb.prot107990 Cold Spring Harb Protoc 2022: pdb.prot107990- This is a work of the US government.
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