A Simple Method for Immunohistochemistry and Imaging of Mosquito Salivary Glands
- Ines Martin-Martin1,
- Thiago Luiz Alves E. Silva1,
- Adeline E. Williams1,2,
- Joel Vega-Rodriguez1 and
- Eric Calvo1,3
- 1Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA
- 2Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
- ↵3Correspondence: ecalvo{at}niaid.nih.gov
Abstract
Immunohistochemistry is a valuable technique that provides information on protein localization and interactions in tissues. Mosquito salivary gland immunohistochemistry requires the meticulous dissection of a delicate tissue. The integrity of the salivary glands must be closely monitored throughout the entire process to prevent structural damage and loss of saliva. This protocol describes a series of simple steps to perform salivary gland immunohistochemistry including tissue dissection, permeabilization, immunostaining, mounting, and imaging by confocal microscopy.
Footnotes
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From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.
