Modified Barth's Solution (MBS) (pH 7.4)
| Reagent | Final concentration (1×) |
|---|---|
| NaCl | 88 mm |
| KCl | 1 mm |
| NaHCO3 | 2.4 mm |
| MgSO4·7H2O | 0.82 mm |
| Ca(NO3)2·4H2O | 0.33 mm |
| CaCl2·2H2O | 0.41 mm |
| HEPES | 5 mm |
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Adjust the pH to 7.4 with NaOH; 3× stocks of MBS are pH-stable and can be stored at 4°C. Prepare a working solution (1/3×) by dilution.
