Recipe

10% Neutral-Buffered Formalin (NBF)

Reagent Quantity
Formaldehyde (37%–40%) 100 mL
Tap H2O 900 mL
Sodium dihydrogen phosphate, monohydrate (NaH2PO4·H2O) 4 g
Disodium hydrogen phosphate, anhydrous (Na2HPO4) 6.5 g
  • Combine the reagents, and confirm that the pH of the solution is between 6.8 and 7.2. Store at room temperature until use.

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  1. doi:10.1101/pdb.rec108006 Cold Spring Harb Protoc 2022: pdb.rec108006- © 2022 Cold Spring Harbor Laboratory Press

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