Recipe

Embryo Lysis Buffer

50 mm Tris pH 8.8

1 mm EDTA

0.5% Tween 20

200 µg/mL Proteinase K (freshly added from a stock solution)

Autoclave and store at room temperature, before adding proteinase K, for up to a year.

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  1. doi:10.1101/pdb.rec107826 Cold Spring Harb Protoc 2022: pdb.rec107826- © 2022 Cold Spring Harbor Laboratory Press
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