Tissue Fixation Solution
| Reagent | Quantity | Final concentration |
|---|---|---|
| Paraformaldehyde (Sigma-Aldrich 158127) | 1 g | 4% |
| 10× PBS (Gibco 2000522) | 2.5 mL | 1× |
| Tween 20 (Fisher 181617) | 75 µL | 0.3% |
-
Combine the first two ingredients and then add the third. Bring the volume to 25 mL with double-distilled H2O. Dissolve by heating at 60°C, and adjust pH to 7.4. Store for up to 2 mo at −20°C.
