2× SSCT
| Reagent | Quantity | Final concentration |
|---|---|---|
| Tween 20 (Fisher 181617) | 75 µL | 0.3% |
| 20× SSC (Invitrogen 00765425) | 2.5 mL | 2× |
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Combine the reagents and then bring the volume to 25 mL with double-distilled H2O. Prepare fresh before use.










