Hybridization Buffer for FISH
| Reagent | Quantity | Final concentration |
|---|---|---|
| Deionized formamide (ThermoFisher AM9344) | 600 µL | 60% |
| Dextran sulfate sodium salt (MilliporeSigma 67578) | 0.1 g | 10% |
| 20× SSC (Invitrogen 00765425) | 100 µL | 2× |
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FISH probe of interest, dissolved in H2O
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Determine the working concentration of the probe empirically, taking into account the brightness of the fluorophore and the affinity and specificity of the probe for the target DNA. Combine the reagents and add double-distilled H2O to 1 mL. Store for up to several months at −20°C.
