Recipe

Embryo Collecting Buffer II

In advance, prepare a 7× stock solution of cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich 11836170001) and a 100 mm stock solution of spermine (e.g., Sigma-Aldrich S1141-1G) in DNase-, RNase-free H2O. Store both at −20°C until use. Prepare the recipe as follows:

2× Stock Solution I 300 µL
1 m DTT (e.g., Sigma-Aldrich 646563, once opened, store at −20°C) 1.2 µL
7× cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail 85.8 µL
100 mm Spermine 0.6 µL
Protector RNase inhibitor (Sigma-Aldrich 3335399001) (40 U/µL) 3 µL
DNase-, RNase-free H2O 209.4 µL

  • Prepare fresh in a 1.5-mL microcentrifuge tube (RNase/DNase-free) and store on ice. This amount of buffer is appropriate for collecting 20 embryos.

| Table of Contents

This Article

  1. doi:10.1101/pdb.rec107966 Cold Spring Harb Protoc 2022: pdb.rec107966- © 2022 Cold Spring Harbor Laboratory Press
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue