Xenopus Nuclear PBS
| 5% BSA (UltraPure, Fisher AM2616, store at −20°C until use) | 680 µL |
| 10× PBS (e.g., Corning 46-013-CM) | 119 µL |
| 1 m MgCl2 (e.g., Sigma-Aldrich M1028-100ML) | 3.4 µL |
| Protector RNase inhibitor (Sigma-Aldrich 3335399001) (40 U/µL) | 8.5 µL |
| DNase-, RNase-free H2O | 889.1 µL |
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Prepare fresh in a 2-mL microcentrifuge tube (RNase/DNase-free) and store on ice.
